Core responsibilities:
• Run routine radioligand binding assay protocols, analyze data & generate weekly reports, carry out tissue culture and cell-based binding assays.
• Fully document all experiments in our electronic laboratory notebook.
Quals--
Qualifications
Education: B.Sc. with 6+ years or M.Sc. with 4+ year or PHD with 3+ years of laboratory experience practicing the required skills.
Must Haves/Required skills:
• Prior experience developing equilibrium and kinetic radioligand binding assays (e.g. 125I, 3H, 35S) in relevant formats is preferred, or willingness to do radioligand work.
• Tissue culture including: (i) cell plating, (ii) cell stimulation and (iii) cell harvesting.
• Experience in membrane preparation
• Experience working with multiwell plates (96- and 384-well formats)
• Note that this position is laboratory-based.
Nice to haves/Preferred skills:
• Experience with liquid handling automation systems (e.g. Echo, Bravo).
• Familiarity with multi-mode plate readers such as the SpectraMax and EnVision Multilabel Reader.
• Exporting and dose-response fitting of data from binding assays.
• Running research operating plan assay and data upload (ROP assay, ActivityBase application)
• Experience with traditional Western blot techniques, including requisite data analysis used to determine abundance of given protein targets. Knowledge of the Protein Simple high-throughput, automated western blotting system is preferred but not required.
Computer/Software skills:
• MS Office suite, Adobe PDF –must have
• Prior experience with an electronic laboratory notebook system is desirable but not required.
• Prior experience with data analysis packages including GraphPad Prism, Activity Base, etc. is desirable but not required.
Personality:
Team oriented, detail oriented, highly collaborative, joyful, and understands timelines.
Note:
• This position is laboratory-based, onsite role.
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